Signalling

Part:BBa_K3697008:Design

Designed by: Lauren Ramlan   Group: iGEM20_Stanford   (2020-10-23)


B. subtilis YFP CDS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 644


Design Notes

Our team required a fluorescent signal protein that could be easily produced and visualized in Bacillus subtilis. Since this YFP can be expressed in both B. subtilis and E. coli, it was easy to see when our vectors were transformed and functioning properly. Additionally, YFP fluorescence could be quantified easily by a plate reader.


Source

Pamela Silver Lab at Harvard Medical School

References