Signalling
Part:BBa_K3697008:Design
Designed by: Lauren Ramlan Group: iGEM20_Stanford (2020-10-23)
B. subtilis YFP CDS
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 644
Design Notes
Our team required a fluorescent signal protein that could be easily produced and visualized in Bacillus subtilis. Since this YFP can be expressed in both B. subtilis and E. coli, it was easy to see when our vectors were transformed and functioning properly. Additionally, YFP fluorescence could be quantified easily by a plate reader.
Source
Pamela Silver Lab at Harvard Medical School